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For H&E staining, the excised tumor and organs were fixed in 4% paraformaldehyde solution, embedded in paraffin, sectioned, and stained with H&E

Rarely, prolonged daily use of other ergot alkaloid drugs (the class of drugs to which dihydroergotamine mesylate nasal spray belongs) has been associated with heart valvular fibrosis

Apart from symptomatic patient care, N -acetylcysteine (NAC) is the only clinically recognized pharmacologic intervention for APAP-overdose patients

The digested samples were lyophilized with a speedvac and resuspended in 0.1% trifluoroacetic acid (TFA) and 5% acetonitrile

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We found that LOsG did not alter the mRNA levels of ER-stress responsive genes Xbp1 and BIP, inflammation-responsive genes IL6 and IL1, and hypoxia-response genes HIF1 and GAPDH compared with sham control mice (Fig