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Diguanylate cyclase activity assay The diguanylate cyclase activity was determined by using both liquid chromatographymass spectrometry (LC-MS) and a pyrophosphate assay

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Comparison with observational cohort data The deCODE study measured 4,907 protein targets in 35,559 Icelanders with the SomaScan v4.0 multiplex aptamer assay and examined the association between plasma protein levels and 373 clinical phenotypes after adjusting for age, sex and multiplicity 23