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Dynamic imaging of cellular pH and redox homeostasis with a genetically encoded dual-functional biosensor, pHaROS, in yeast

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Hybridoma cells were subcloned five limiting dilution rounds to obtain the stable monoclonal cell clone MS4A 5E6 (rat IgG2c/)

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Its involvement in sleep-associated hormonal rhythms makes DSIP particularly relevant for studying the relationship between circadian biology and endocrine signaling