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Advanced imaging techniques, including confocal imaging, and high-throughput data acquisition with microplate readers, further support the validation process by enabling precise, real-time monitoring of roGFP fluorescence in different cell types and cellular compartments

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A few commenters maintained that they expect to spend $20,000-$30,000 to elicit vendor support to encode the new data elements in the MRF by the January 1, 2026, effective date proposed in the CY 2026 OPPS/ASC proposed rule

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