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In the most widely held model, CFTR activation depends on release of the R-domain positioned between NBD1 and NBD2 upon phosphorylation to induce a completely unbound and disordered state that releases the NBDs to allow dimerization 26

They stress that there is little evidence of benefit for healthy people and that the treatments can be very expensive

After 30 min incubation, cells were added (500,000 for DMSO and 800,000 for etoposide-treated cells) in DMEM media supplemented with only 10% FBS (no antibiotics)

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Deacon CF, Johnsen AH, Holst JJ

They got her to calm down and she was still ticking and stuff