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Cell membranes were solubilized in solubilization buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 1% sodium cholate, 0.05% DDM, 5 mM MgCl 2 , 2 L CIP, 5 mM -ME, 10 M GDP, 10% glycerol, 0.2 g/ml leupeptin and 150 g/ml benzamidine