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Analytical Method & Instrumentation Method:ICP-MS per ICH Q3D(R2) / USP / ChP 0412 Instrument:Agilent 7900 ICP-MS (SN: JP29457) with He collision mode Sample Prep:Closed-vessel microwave digestion (CEM MARS 6): 13.7 mg + 5mL HNO(suprapure) + 1mL HO (30%) Digestion:Ramp 20min 200C, hold 15min, cool to RT, dilute to 50.0 mL (Milli-Q, 18.2 Mcm) Calibration:Multi-element std (NIST SRM 3100 series), 5-point external cal

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Tirzepatide achieved 20.9% weight loss vs semaglutide's 16.0% at equivalent doses, and 52% of tirzepatide users lost 15% or more body weight vs 35% of semaglutide users

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had obesity from August 2021 to August 2023