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A drop of 150 nl enzyme/cofactor/substrate solution was mixed with 150 nl precipitant (100 mM MES, pH 6.4, 200 mM MgCl 2 , 17.9% PEG200) and equilibrated against 50 l reservoir solution (100 mM MES, pH 6.4, 200 mM MgCl 2 , 17.9% PEG200)

13 C NMR (DMSO d 6 , 125 MHz): c (ppm) = 163.9, 159.7, 150.4, 147.7, 129.2, 125.9, 124.8, 121.6, 121.3, 120.0, 116.2, 115.4, 111.9, 109.7, 56.2