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copper complexation is typically performed by mixing the tripeptide with copper(II) salts (chloride, acetate, or sulfate) at controlled pH, with the resulting complex purified by chromatography

This advanced peptide compound consists of 83 amino acids, featuring a 13 amino acid N-terminal extension at the N-terminus and an arginine substitution at position 3, changes that fundamentally alter its natural properties compared to native IGF-1