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Research Methodology 12.2

Copper-free GHK can chelate copper from surrounding media or biological fluids, making its effects dependent on ambient copper availability, which introduces an uncontrolled variable in experimental systems

Formalin-fixed and paraffin-embedded (FFPE) primary breast tumor samples and endocrine therapy resistant breast tumor samples were from the Pathology Department at The First Affiliated Hospital of Wenzhou Medical University and The Third Affiliated Hospital of Wenzhou Medical University