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RNA Scope Hypothalamic cultures (HUES-POMC-GFP and KOLF2.1J-POMC-NG) at 40 days post-differentiation were dissociated, washed with Dulbeccos Phosphate-Buffered Saline (DPBS -/- ), and approximately 10 million cells per cell line were fixed in 10% neutral buffered formalin for 4 hours at room temperature on a rotating mixer

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C.AnginM.VolantS.PassaesC.MonceauxV.MikhailovaA.et al

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