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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions
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Invitrogen) followed by injection of inactive Exendin-4 (E9)-Cy3 (50l, 0.5-2nmol in HBSS) via the tail vein
For comparison, the modified GHRH analog CJC-1295 (specifically the version without DAC, which is what is commonly used in modern clinics) has a longer functional duration that keeps the GHRH stimulus present long enough for a more meaningful, sustained growth hormone pulse to occur
Complete Herbal Tutor : The Definitive Guide to the Principles and Practices of Herbal Medicine (Second Edition)
