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LAG-1 and other CSL proteins share the same in vitro DNA binding site (GTGGGAA, LAG-1/CSL binding site hereafter) [16,18,19]

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Here, we benchmarked several engineered laccase templates 22,23,24,25 , identified an ancestral fungal variant with slight activity in HEK293T cells and performed extensive directed evolution on the yeast surface to improve its activity (Fig