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ThermoFisher, 11320033) with 2 mM L-glutamine, 10 mM HEPES (Sigma-Aldrich, H3375-25g), 50 U ml 1 penicillinstreptomycin, 1 B27 (ThermoFisher, 12587-010), 1 N-2 (ThermoFisher, 17502-048), 50 ng ml 1 EGF (Peprotech, AF-100-15) and 100 ng ml 1 Noggin (Peprotech, 250-38)

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Ingredient Quantity Cyanocobalamin (Vitamin B12) 1000 mcg/mL Sodium Chloride 0.22% w/v Ammonium Sulfate 0.1% w/v Citric Acid 0.01% w/v Sodium Citrate 0.008% w/v Benzyl Alcohol (Preservative) 1.0% v/v

GIP receptor activation enhances insulin sensitivity at the cellular level