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The sodium chloride concentration is identical to standard preservative-free saline isotonic, 9 mg NaCl per mL

A., Yang, H., Water, B

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[cited 2/7/22]

Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)