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These effects are diminished or lost in 3-KO mice, indicating that 3-AR signaling is essential for many, but not all, of AOD9604s actions

In preclinical heart failure models, NNMT enzyme inhibition markedly reduced cardiac 1-MNA (by 77.1%), improved cardiac function, reduced left ventricular hypertrophy and fibrosis, and decreased pro-inflammatory gene expression

The compounds bind to entirely different receptors (beta-3 adrenergic vs GLP-1/GIP)

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This matches the 20mg vial sweet spot (200mcg per unit) while providing 2.5 weeks of peptide at the maximum 12mg dose