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The fluorescence intensity of each protein was calculated as the product of the average fluorescence intensity and fluorescence area

Acknowledgments This project was funded by the National Plan for Science Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology

It enhances mineralization and stimulates growth factor expression in fracture or cartilage injury models, making it valuable for orthopedic and degenerative joint research

Our results are in line with previous reports emphasising that the available ELISA kits to quantify BDNF isoforms, especially mBDNF, need to increase their specificity to be suitable as biomarkers in biomonitoring programmes as well as in clinical applications 31,32

doi: 10.1038/s41598-021-86425-6

Because B12 is mostly in animal products, Vegans are at risk, as their diet is plant-based and they should look for foods fortified with vitamin B12