Duvvuri S, v R, Mak DH, Kojima K, Konopleva M, Andreeff M , Borthakur G
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At the end, separated proteins were transferred to fluorescence-adapted polyvinylidene difluoride (PVDF) membrane (Immobilon FL-Membrane, Merck Millipore) using the following buffer (25 mM Tris-Base, 192 mM glycine, 0.0125% SDS and 20% methanol) at 400 mA for 60 min