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Right upper quadrant pain, fever, jaundice, or dark urine could indicate gallbladder problems and require immediate attention

These factors may seem unrelated to peptide dosing, but they substantially impact outcomes

Finally, given the pragmatic nature of the proposed trial, the decision was taken to include consecutive patients seeking medical help at the participating centres, thus ensuring the enrolment of subjects similar to those that would be seen in normal clinical practice

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This includes applying an ice pack for 10-20 minutes around the injection site

Here, we developed a Si ngle- M olecule C olocalization A ssay (SiMCA) that overcomes this problem by employing total internal reflection fluorescence microscopy to quantify target proteins based on the colocalization of fluorescent signal from orthogonally labeled capture and detection antibodies