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The column was pre-equilibrated with 10 CV of DPBS (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant adjusted to pH 7.2 was loaded onto the column

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This pulsatile nature is considered central to its physiological effectiveness

Last Updated Saturday, August 9, 2025, 06:15 PM BST The content of this article is for informational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment