It is accomplished via continuous provision of glutathione precursors, especially cysteine, during splenic lymphocyte proliferation
10.1016/j.bbi.2020.03.014 82
10.1111/j.1600-0625.2009.00957.x 23 ChenX.JinW.ChenD.DongM.XinX.LiC.et al (2021)
Isolated MM cells were cultured in Iscoves modified Dulbecos medium supplemented with 20% FBS, insulin, selenium, transferrin, 10 g/ml of gentamycin, 10 ng/ml of interleukin-6, insulin-like growth factor-1 and vascular endothelial growth factor
Cells were washed, colleted with 1 mM EDTA in PBS, pelleted (500 g for 5 min) and re-suspended in isolation medium (320 mM sucrose, 10 mM TrisHCl, 1 mM EDTA, pH 7.4)
doi:10.12659/MSM.934914 125