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All surface areas were calculated based on the crystal structures using freeSASA 48

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This indicates that the stability of the mutant systems is relatively higher than that of the native GLP-1-containing system

Purification of GLP-1RPF-06372222Fab7F38 complex The 1 L cell biomass expressing modified GLP-1R construct was lysed in a low salt buffer containing 10 mM HEPES pH 7.5, 20 mM KCl, 10 mM MgCl 2 , and EDTA-free protease inhibitor cocktail tablets