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J744A.1 mouse macrophage cells were obtained from the American Type Culture Collection (ATCC)

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Orthosteric binding of incretin peptides to the N-terminus (extracellular) region of the receptors causes a conformational change, stabilizing a binding site in the intracellular region that allows coupling to and activation of heterotrimeric guanine nucleotide-binding protein (G proteins) ( a and subunits to dissociate triggering different signalling cascades

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keep solution at 28 C for up to 7 days to maintain 98% integrity