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Attaching a fatty acid via a chemical linker to a specific residue on an already-assembled peptide requires controlling the proportion of conjugated versus unconjugated species, detecting and controlling free fatty acid or linker impurities, and monitoring for di- or poly-modification at unintended sites

A significant driver of dysautonomia is small fiber neuropathydamage to the tiny, unmyelinated or thinly myelinated nerve fibers that control involuntary autonomic functions like heart rate, blood pressure, and digestion