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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

Another tumor to be considered in the differential diagnosis in our patients was malignant melanoma because some cells were found to contain melanin on microscopic inspection

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Summary Studies have shown a significant role of angiogenesis in the pathophysiology of neurodegenerative diseases

These aim to complementother aspects of a weight loss regimen, including exercise and a low calorie diet