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A: Yes, pairing them is highly recommended

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Together, our findings indicate that LaccID targets cleanly to the cell surface and is selectively active there, in contrast to APEX2 and HRP, which exhibit activity in ER and Golgi compartments

References Witschi A, Reddy S, Stofer B, Lauterburg BH

The improvement in maximal walking distance was 123 versus 54% (p = 0.004)

The mitochondrial metabolism generated a considerable amount of ROS like H2O2, hydroxyl radicals, superoxide, etc