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As pyridoxal is the only vitamin B6 form that can cross the BBB [185], PLP needs to be hydrolyzed to pyridoxal by tissue non-specific alkaline phosphatase (TNAP)

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Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

Formulation matters as much as timing

What is measured in these rats and mice (and occasionally in small groups of human volunteers) is often not a hard endpoint like muscle strength or weight loss, but surrogate biomarkers: molecules in the blood that have an indirect link to a hard endpoint