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In brief, 200 L of a serum-free medium containing 5 10 4 cells was added to the upper chamber with the Corning BioCoat Matrigel (Corning Life Sciences, New York, USA) and allowed to invade toward the lower chamber with 10% FBS

doi: 10.1056/NEJMoa1611977 7 McCutcheonIEPreulMC

Further validation of the molecular mechanisms was performed using flow cytometry, Western blotting, and reverse transcription quantitative polymerase chain reaction (RT-qPCR)

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on his initiative, the National Research Program (NRP) 74 was established for the Health Services Research area in Switzerland in 2017