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Separation of the digests was achieved by employing a C18 EASY-Spray HPLC column (75 m 500 mm, 2 m, ES903 Thermo Fisher Scientific) with a linear gradient ranging from 4% to 90% acetonitrile at a flow rate of 300 nL per minute (nL/min) over a duration of 150 min

253 The methylation of histones is a complicated process that controls the dynamics of chromatin and the transcription of genes

Choi HI, Yang DM, Kim HC, Kim SW, Jeong HS, Moon SK, et al

However, Massi et al