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[23] Research indicates that butyrate may influence sleep through: Modulation of vagal tone, promoting parasympathetic "rest and digest" activity Regulation of cortisol rhythms via the HPA axis Support for melatonin signaling pathways Reduction of inflammation that can interfere with sleep quality People with adequate butyrate levels have been associated with better sleep quality and more consistent energy throughout the day, though these effects likely result from butyrate's combined actions rather than any single mechanism

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The co-culture system was grown in a humidified incubator at 37 C and 5% CO 2 for 48 h

Endocrinology , 142(12), 5182-5189

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