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Editing efficiency was determined on bulk-edited cell populations by targeted PCR of the Cas9-targeted region and secondary PCR using overhang sequences (Table S1), followed by Illumina MiSeq, as previously described (Aubrey et al, 2015)

As the skin ages, the ECM gradually degrades and deteriorates, leading to noticeable changes in skin function and appearance [41, 42]

Xeomin (or incobotulinumtoxina) is a type of botulinum toxin type A

The mice bearing the MC38-hPD-L1 tumor model were acclimated at 25 C and 55% of humidity under natural light/dark conditions, with standard mice chow and water available ad libitum

10.1016/j.semcancer.2019.08.028 33 KongH.ChuQ.FangC.CaoG.HanG.LiX

This section summarizes various compounds that can suppress ferroptosis and their roles in the metabolic and molecular pathways associated with ferroptosis