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Silvestrini et al., 2006) and neurovascular coupling responses and severity of cognitive impairment in individuals with MCI and ADRD (van Dijk et al., 2024)

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10.1016/j.nrleng.2018.08.004 27 RiemannD.KroneL

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Cells were subsequently blocked by 2% BSA in PBS-T (0.1% Tween 20 in PBS) for 1 h at room temperature, incubated with the primary antibody Anti-Rheb (Santa Cruz Biotechnology Cat# sc-271509, RRID: AB_10659102, 1:500) overnight at 4 C in blocking buffer, then washed with PBS-T three times and incubated with Alexa-555-conjugated secondary antibody (Invitrogen # A-31570, RRID:AB_2536180, 1: 1000) and DAPI (1 g/ml) in blocking buffer for 1 h at room temperature with protection from light

Slide needle along side of tendon, taking care not to enter into tendon itself, Deposit half of the solution while slowly withdrawing needle, Insert needle on lateral side and repeat procedure with remaining solution