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Aliquots of 20 l were taken from every pH value at different time points (1, 4, 48, 96 and 144 h) and added over 180 l of 20 mM Britton and Robinson buffer at pH 7.0, which was preloaded in a flat-bottom 96-well microtiter plate (Greiner Bio-One, 655101)

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Less common: Mild nausea, headache, or temporary fatigue

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Symptoms of growth hormone deficiency also depend on age, and often those meeting the classic definition of adult onset GHD can have different symptoms than a child similarly diagnosed