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In experiments aiming to capture more interacting proteins, the beads were washed five times with 0.5 ml of cold lysis buffer (50 mM Tris-HCl, 0.8% Igepal-CA630, 5% glycerol, 150 mM NaCl, 1.5 mM MgCl2, pH 7.5, 1 mM DTT, cOmplete mini protease EDTA-free inhibitor) containing additional 0.2% NP-40, referred to as soft wash in this manuscript
& Herrera-Acosta, J
Lintner K, Peschard O
Gallbladder disease : GLP-1 receptor agonists as a class are associated with an increased risk of cholelithiasis (gallstones) and cholecystitis
doi: 10.1038/s41598-019-40354-7 100 GotsmanIMuszkatM
Khng c ung thuc khi pht hin bt thng
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