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the column was washed with 20 ml equilibration solution, and elution was performed with a linear pH gradient from 3.2 to 8.0 (1.54 % (w/w) tri-sodium citrate, 0.6 % (w/w) succinic acid, 1.09 % (w/w) di-sodium hydrogen phosphate di-hydrate, pH 8.0) followed by 40 ml of isocratic elution at pH 8.0

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Pro-gram Project from the State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou Medical University (Grant No