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Following a final saline wash, 50% glycerol was added to the wells, and bright-field images were taken using an inverted microscope

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they may either interfere with the binding of the virion to the cellular receptors, fusion with the host membrane in case of enveloped viruses, membrane penetration (for nonenveloped viruses), may block uptake into the cells, prevent uncoating of the genomes in the endosomes or cause aggregation of virus particles 37