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In the Eqn.4 L is the concentration of the competing ligand, K 1 and K 2 correspond to the dissociation constants of CuL and CuL 2 complexes, respectively [Cu] total is the concentration of copper and [Cu] bound to protein is the concentration of the copper bound to the protein at IC50

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Individuals considering this treatment should consult with healthcare providers to thoroughly understand the implications of its use and explore all possible options to make an informed decision about their health management strategy.