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Transmission electron microscopy (TEM) (Figure 2) and atomic force microscopy (AFM) assess particle size and morphology (110 nm), while X-ray diffraction (XRD) reveals partial graphitisation with broad peaks near 24 [ 1 ) and G (1,580 cm 1 ) bands, with I_D/I_G ratios which quantify the degree of structural disorder or defects in the carbon lattice [Figure 2) [ FIGURE 2 Overall, the combined influence of nanoscale structure, doping, and surface passivation governs the performance of CQDs

After 72 h, cells were harvested, washed, and lysed in lysis buffer and Western blot analysis was performed

doi: 10.1093/aje/154.6.495 91

participant characteristics including sample size, age, and diagnostic criteria

Supplementing with targeted peptides helps reignite these essential biological messages

A clinician review step is consistent with best practice