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Plan your experiments to minimize how often stored peptides are accessed

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Plotted was the normalized enrichment (log2) of replicate 1 against replicate 2 with swapped SILAC labels

Peptides were initially desalted in-line using an Acclaim PepMap C18 trap column (75 m 2 cm) (Thermo Fisher Scientific) for 2 min at a flow rate of 5 L min 1 with 2% acetonitrile (ACN) and 0.2% formic acid (FA)

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