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In the next step, this bisulfite converted DNA was amplified using Pyromark PCR kit (Qiagen cat#978703) where the PCR conditions were 1 cycle for 15 min at 95 C and next 40 cycles for 30 s at 94 C, 30 s at 56 C and 10 min at 72 C

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Muscle preservation: AOD 9604 does not affect muscle