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hNF-1 was grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (low glucose with l-glutamine and phenol red, Sigma-Aldrich Co., Llc.) and 5 vol% FBS (Thermo Fisher Scientific Inc.)

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In rats with experimental AIT, after the use of selenium yeast dietary supplement, in addition to a decrease in the levels of TgAb, TPOAb and a decrease in B-cell-activating factor (BAFF) in the blood serum, the expression of IL-10 in the thyroid tissue increased, the number of Breg in the spleen increased, and also decreased TSH level (77)

Free Radic Biol Med 237, 570584.Google Scholar Thomas, TA, Francis, RO, Zimring, JC et al