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Otherwise, surgeons may opt for a limb-saving treatment, by removing less of the surrounding tissue or part of the bone, which is replaced by a metal rod or grafts

2.5 Hematological analyses After 8 weeks of administration, the remaining 40 rats were anesthetized with isoflurane, then blood was collected in a heparin sodium tube, and the left hind limb was amputated at 1 cm above the ankle joint

This "low and slow" approach gives your microbiome and stomach acid levels time to adjust to the new protein load