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We have previously shown that, in the region surrounding the NPEY motif, 4 out of 16 residues differ between the IR and IGF1R (equivalent to Pro963, Ser968, Leu973, and Ser976 in the IR) and by systematic substitution of each of these residues showed that only replacement of leucine-973, the residue in the +1 position following the NPEY motif, with phenylalanine results in a shift from binding IRS-1 to binding Shc in a receptor pull-down assay (14)

Marketou ME, Maragkoudakis S, Anastasiou I, et al

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The authors concluded that supplementation of HA to collagen did not improve various skin parameters, and therefore, the dermal benefits of the HA active ingredient could not be confirmed in this study [53]