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Common observations during this period include skin texture feeling slightly less plump or firm, fine lines that had completely disappeared beginning to reappear subtly, and overall skin quality remaining better than pre-treatment but noticeably less optimal than peak treatment results

Cells were cultured in 200 L Neurobrew (Milteny Biotec, Bergisch Gladbach, Germany), 100 L N2-supplement (Thermo Fisher Scientific, Waltham, MA), 2 L bFGF (PeproTech, Princeton, NJ) and 2 L PDGF-BB (R&D Systems, Minneapolis, MA) per 10 mL of DMEM/F-12 media (Thermo Fisher Scientific, Waltham, MA)

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Moreover, metformin treatment prevented the degradation of cartilaginous proteoglycans and collagen, reduced cartilage erosion, and caused lower OARSI scores compared with the OA model group (Fig