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The qRT-PCR reaction was made up of 4.5 L diluted (20X) cDNA template, 0.25 L forward and 0.25 L reverse primers of GSTs, and 5 L of Maxima SYBR Green qPCR Master Mix (2X) (Thermo Scientific, Waltham, MA, USA) in double-distilled water at a final volume of 10 L

Anxiety disorders affect about 30% of adults Richard Maddock, senior author of the study, is a psychiatrist and research professor in the Department of Psychiatry and Behavioral Sciences

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A detailed health history and symptom review are also key parts of identifying a leaky gut

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