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It has been used in experimental models to examine cortisol levels, hypothalamic-pituitary axis activity, and sleep structure

It also plays a key role in the immune system

Once opened, it should be considered contaminated and must be discarded, as refrigeration alone isnt sufficient for a multi-use vial

Alamdari, N., et al

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma

Immunoprecipitation - Cecum, liver, and brain tissues were collected in IP lysis buffer (150 mM NaCl, 10 mM Tris-HCl, pH 7.4, 1% NP-40, 1 mM PMSF, and 1 mM protease inhibitor cocktail), homogenized, and incubated on ice for 20 min to ensure complete lysis