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For tyrosinase activity, the cells were lysed in 100 L of 50 mM sodium phosphate buffer (pH 6.5) containing 5 L of 1% Triton X-100 and 5 L of 0.1 mM phenylmethylsulfonylfluoride
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control on the occurrence of symptomatic cholelithiasis
Importantly, it also involves the braingutimmunepain axis , a bidirectional network through which the central nervous system, gut, immune cells, and peripheral nerves communicate