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Sometimes because the biology doesn't translate

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2.8 Proteomic and metabolomic analyses 2.8.1 Sample preparation In postoperative day 7, hippocampal tissues were dissected on ice using sterile instruments ( n = 6 for each groups), rinsed with 0.9% saline, and placed into pre-chilled cryotubes for immediate liquid nitrogen freezing

Neuron 69 , 780792 (2011)