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Following neutralization with (NH) 4 HCO 3 , samples were frozen at 20 C for 20 min, spun and supernatants measured directly

Gclc f/f mice 27 were crossed with the MMTV-PYMT (The Jackson Laboratory, 022974) 61 and Rosa26 creERT2 (The Jackson Laboratory, 008463) 62 mouse strains

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Data processing utilized the SUSHI pipeline for alignment, read counting, and quality control

These findings underscore the urgent need for targeted antioxidant therapies capable of quenching this systemic ROS burden and restoring metabolic homeostasis

Glutathione regulates the expression of gamma-glutamylcysteine synthetase via the Met4 transcription factor