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For IL-1 experiments, THP-1 cells (1 10 6 per well) were seeded in a 12-well plate and differentiated overnight with 10 nM phorbol myristate acetate

The mechanism relates to ghrelin receptor activation

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Wang DY, McQuade JL, Rai RR, Park JJ, Zhao S, Ye F, Beckermann KE, Rubinstein SM, Johnpulle R, Long GV, Carlino MS, Menzies AM, Davies MA , Johnson DB